Summary
This study identifies 117 light-regulated genes in zebrafish, revealing molecular pathways by which light entrains circadian clocks at the organism, organ, and cellular levels. The findings suggest D-box regulatory elements represent a general mechanism for light-induced gene expression, with implications for understanding how spectral properties of light drive circadian entrainment.
Key Findings
- 117 light-regulated genes were identified in zebrafish larvae, heart organ cultures, and cell cultures exposed to 1- or 3-hour light pulses.
- Genes clustered into five major classes showing regulation at all organizational levels or in different subset combinations.
- Gene ontology analysis revealed enrichment of genes involved in circadian rhythms, stress response, and DNA repair.
- D-box regulatory elements were identified as general mediators of light-induced gene expression, not limited to the period2 gene.
- Morpholino knockdown of two Tef homologues (D-box binding factors) showed gene-specific differential involvement in cell-autonomous light induction.
Categories
Sleep & Circadian Health: Study investigates light-induced entrainment of circadian clocks at multiple organizational levels, identifying genes regulated by light exposure.
The Science of Light: Characterizes wavelength-dependent photoreception and the molecular mechanisms by which light induces gene transcription relevant to circadian regulation.
Author(s)
MF Haug, V Lazovic, SCF Neuhauss
Publication Year
2012
Related Publications
Sleep & Circadian Health
- Phototransduction by retinal ganglion cells that set the circadian clock
- The mammalian circadian timing system: organization and coordination of central and peripheral clocks
- The two‐process model of sleep regulation: a reappraisal
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The Science of Light
- Phototransduction by retinal ganglion cells that set the circadian clock
- Color appearance models
- The mammalian circadian timing system: organization and coordination of central and peripheral clocks
- Diminished pupillary light reflex at high irradiances in melanopsin-knockout mice
- Melanopsin is required for non-image-forming photic responses in blind mice