Summary
This study characterizes the expression of melanopsin (Opn4) and circadian clock genes (Clock, Cry, Per) in zebrafish embryonic ZEM-2S cells under light-dark cycles, constant darkness, and melatonin pulse conditions. Melanopsin mRNA showed two expression peaks under light-dark cycles that persisted in constant darkness, while Clock and other clock genes showed limited or no clear rhythmicity in these cell cultures.
Key Findings
- Melanopsin expression peaked at ZT3 and ZT12 under 12:12 light-dark cycles, with both peaks maintained in constant darkness.
- MT2 melatonin receptors (but not MT1) were identified in ZEM-2S cells, suggesting a specific melatonin signaling pathway.
- All six known zebrafish Cry genes were expressed in ZEM-2S cells, but Clock mRNA showed no clear rhythmicity under any tested condition.
Categories
The Science of Light: Investigates melanopsin and circadian gene expression regulation by light cycles and melatonin in zebrafish cells
Sleep & Circadian Health: Examines core clock gene rhythmicity (Clock, Cry, Per) under light-dark cycles and constant darkness
Author(s)
CBLB Martins
Publication Year
2007
Number of Citations
1
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