Summary
This study examines molecular targets for promoting retinal ganglion cell survival and optic nerve regeneration after injury, using siRNA-mediated knockdown of GSK3β and RTP801 in rat models. While not directly applicable to lighting design, findings on RGC survival mechanisms are relevant background for understanding photoreceptor and ganglion cell health that underlies light sensing and circadian signaling.
Key Findings
- siGSK3β knockdown significantly promoted RGC survival and increased the number of RGCs with neurites in vitro, an effect sensitive to Rapamycin (mTOR pathway dependent).
- GSK3β knockdown overcame Nogo-A-mediated neurite growth inhibition in vitro.
- In vivo, siGSK3β enhanced RGC survival after optic nerve crush but did not increase axon number or length alone.
- Combined knockdown of GSK3β and RTP801 significantly increased RGC survival, neurite outgrowth, and axon regeneration beyond either treatment alone.
Categories
Eye Health & Vision: Directly investigates retinal ganglion cell (RGC) survival and axon regeneration after optic nerve crush, relevant to retinal and optic nerve health.
Author(s)
Z Ahmed, PJ Morgan-Warren, M Berry, RAH Scott
Publication Year
2019
Number of Citations
10
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